Efficient immobilization of agarase using carboxyl-functionalized magnetic nanoparticles as support

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• 作者：Anfeng Xiao^a ; ^b ; ¹ ; Qiong Xiao^a ; ¹ ; Yan Lin^c ; Hui Ni^a ; ^b ; ^{nihui@jmu.edu.cn} ; Yanbing Zhu^a ; ^b ; Huinong Cai^a ; ^b
• 关键词：Agar-derived oligosaccharides ; Agaro-oligosaccharide ; Dynamic light scattering ; Enzyme characterization ; Scanning electron microscopy ; Thermogravimetric analysis ; Transmission electron microscopy ; Vibrating sample magnetometry ; X-ray diffraction ; Zeta-potential analysis
• 刊名：Electronic Journal of Biotechnology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：25
• 期：Complete
• 页码：13-20
• 全文大小：1372 K
• 卷排序：25

文摘

A simple and efficient strategy for agarase immobilization was developed with carboxyl-functionalized magnetic nanoparticles (CMNPs) as support. The CMNPs and immobilized agarase (agarase-CMNPs) were characterized by transmission electron microscopy, dynamic light scattering, vibrating sample magnetometry, scanning electron microscopy, X-ray diffraction, thermogravimetric analysis, and zeta-potential analysis. The hydrolyzed products were separated and detected by ESI-TOF-MS.ResultsThe agarase-CMNPs exhibited a regular spherical shape with a mean diameter of 12 nm, whereas their average size in the aqueous solution was 43.7 nm as measured by dynamic light scattering. These results indicated that agarase-CMNPs had water swelling properties. Saturation magnetizations were 44 and 29 emu/g for the carriers and agarase-CMNPs, respectively. Thus, the particles had superparamagnetic characteristics, and agarase was successfully immobilized onto the supports. Agaro-oligosaccharides were prepared with agar as substrate using agarase-CMNPs as biocatalyst. The catalytic activity of agarase-CMNPs was unchanged after six reuses. The ESI-TOF mass spectrogram showed that the major products hydrolyzed by agarase-CMNPs after six recycle uses were neoagarotetraose, neoagarohexaose, and neoagarooctaose. Meanwhile, the end-products after 90 min of enzymatic treatment by agarase-CMNPs were neoagarobiose and neoagarotetraose.ConclusionsThe enhanced agarase properties upon immobilization suggested that CMNPs can be effective carriers for agarase immobilization. Agarase-CMNPs can be remarkably used in developing systems for repeated batch production of agar-derived oligosaccharides.