An omics approach to rational feed: Enhancing growth in CHO cultures with NMR metabolomics and 2D-DIGE proteomics
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- 作者：Eric J.M. Blondeela ; Raymond Hob ; Steffen Schulzea ; c ; Stanislav Sokolenkoa ; Simon R. Guillemettea ; Igor Slivacd ; Yves Durocherd ; J. Guy Guillemettee ; Brendan J. McConkeyb ; David Changf ; Marc G. Aucoina ; marc.aucoin@uwaterloo.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：CHO cells ; 2D-DIGE ; Proteomics ; NMR ; Metabolomics
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 September 2016
- 年：2016
- 卷：234
- 期：Complete
- 页码：127-138
- 全文大小：2129 K
文摘
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Expression of recombinant proteins exerts resource stresses on cell culture systems.
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Between parental cell line and antibody-producing progeny, 34 differentially expressed proteins were identified by 2D-DiGE.
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50 nutrients and metabolites tracked by 1D-1H-NMR metabolomics.
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Stresses linked to TCA-cycle, THF cofactor conversions, limits to lipid synthesis.
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8 nutrients added to medium achieved a ∼75% improvement to peak cell densities.