Application of stable isotopes to investigate the metabolism of fatty acids, glycerophospholipid and sphingolipid species

详细信息    查看全文

• 作者：Josef Ecker ; Gerhard Liebisch ; ^{gerhard.liebisch@klinik.uni-regensburg.de" class="auth_mail}
• 关键词：BMP ; bis(monoacylglycero)phosphate ; Cer ; ceramide ; CoA ; Coenzyme A ; Des ; desaturation ; DG ; diacylglycerol ; DihCer ; dihydroceramide ; DiSPH ; dihydrosphingosine (sphinganine) ; DMDS ; dimethyl disulfide ; ESI-MS/MS ; electrospray ionization tandem mass spectrometry ; FA ; fatty acid ; FADS ; fatty acid desaturase ; FAME ; fatty acid methyl ester ; FASN ; fatty acid synthase ; FT-ICR ; Fourier transform ion cyclotron resonance ; GC&ndash ; IRMS ; gas chromatography isotope ratio mass spectrometry ; GlcCer ; glucosylcer
• 刊名：Progress in Lipid Research
• 出版年：April, 2014
• 年：2014
• 卷：54
• 期：Complete
• 页码：14-31
• 全文大小：3010 K

文摘

Nature provides an enormous diversity of lipid molecules that originate from various pathways. To gain insight into the metabolism and dynamics of lipid species, the application of stable isotope-labeled tracers combined with mass spectrometric analysis represents a perfect tool. This review provides an overview of strategies to track fatty acid, glycerophospholipid, and sphingolipid metabolism. In particular, the selection of stable isotope-labeled precursors and their mass spectrometric analysis is discussed. Furthermore, examples of metabolic studies that were performed in cell culture, animal and clinical experiments are presented.