Isolation and characterization of genes encoding leucoanthocyanidin reductase (FeLAR) and anthocyanidin reductase (FeANR) in buckwheat (Fagopyrum esculentum)

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• 作者：Katsuhiro Matsui^a ; ^{matsuik@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Tomomi Hisano^a ; Yasuo Yasui^b ; Masashi Mori^c ; Amanda R. Walker^d ; Toshikazu Morishita^e ; Kenjiro Katsu^a
• 关键词：ANR ; anthocyanidin reductase ; ANS ; anthocyanidin synthase ; BGDB ; buckwheat genome database ; bHLH ; basic Helix-Loop-Helix ; CHI ; chalcone isomerase ; CHS ; chalcone synthase ; DFR ; dihydroflavonol 4-reductase ; DMACA ; 4-dimethylaminocinnamaldehyde ; DW ; dry weight ; F3H ; flavanone 3-hydroxylase ; F3&prime ; H ; flavonoid 3&prime ; -hydroxylase ; F3&prime ; 5&prime ; H ; flavonoid 3&prime ; 5&prime ; -hydroxylase ; FLS ; flavonol synthase ; GT ; glucosyl transferase ; LAR ; leucoanthocyanidin reductase ; PA ; proanthocyanidin
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：20 October 2016
• 年：2016
• 卷：205
• 期：Complete
• 页码：41-47
• 全文大小：1458 K

文摘

Proanthocyanidins (PAs) are a major group of flavonoids synthesized via the phenylpropanoid biosynthesis pathway, however the pathway has not been fully characterized in buckwheat. Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) are involved in the last steps of PA biosynthesis. To isolate the genes for these enzymes from buckwheat we performed PCR using degenerate primers and obtained cDNAs of ANR and LAR, which we designated FeANR and FeLAR1. A search for homologs in a buckwheat genome database with both sequences returned two more LAR sequences, designated FeLAR2 and FeLAR3. Linkage analysis with an F₂ segregating population indicated that the three LAR loci were not genetically linked. We detected high levels of PAs in roots and cotyledons of buckwheat seedlings and in buds and flowers of mature plants. FeANR and FeLAR1–3 were expressed in most organs but had different expression patterns. Our findings would be useful for breeding and further analysis of PA synthesis and its regulation in buckwheat.