文摘
F2H AR is a novel live-cell assay for evaluation of androgens and antiandrogens. DHT, abiraterone, bicalutamide, enzalutamide, flutamide, galeterone were tested. W741L, F876L, T877A and F876L/T877A AR mutations were evaluated with F2H AR. The assay enables real-time visualization and monitoring of compounds’ activity. The assay facilitates comparative analysis of mutant AR activation upon treatments.