Endogenous cytokinin profiles of tissue-cultured and acclimatized 鈥榃illiams鈥?bananas subjected to different aromatic cytokinin treatments
详细信息 查看全文
- 作者：Adeyemi O. Aremu ; Lenka Pla膷kov谩 ; Michael W. Bairu ; Ond艡ej Nov谩k ; Lucie Sz眉膷ov谩 ; Karel Dole啪al ; Jeffrey F. Finnie ; Johannes Van Staden
- 关键词：BA ; N6-Benzyladenine ; BA9G ; N6-Benzyladenine-9-glucoside ; BAR ; N6- Benzyladenine riboside ; BAR5&prime ; MP ; N6-Benzyladenosine-5&prime ; -monophosphate ; CDK ; Cyclin-dependent kinase ; CK ; Cytokinin ; cZ ; cis-Zeatin ; cZ9G ; cis-Zeatin-9-glucoside ; cZOG ; cis-Zeatin-O-glucoside ; cZR ; cis-Zeatin riboside ; cZR5&prime ; MP ; cis-Zeatin riboside-5&prime ; -monophosphate ; cZROG ; cis-Zeatin-O-glucoside riboside ; DHZ ; Dihydrozeatin ; DHZ9G ; Dihydrozeatin-9-glucoside ; DHZOG ; Dihydrozeatin-O-glucoside ; DHZR ; Dihydrozeat
- 刊名：Plant Science
- 出版年：January, 2014
- 年：2014
- 卷：214
- 期：Complete
- 页码：88-98
- 全文大小：1444 K
文摘
Endogenous cytokinin (CK) levels of in vitro-cultured and greenhouse-acclimatized 鈥榃illiams鈥?bananas treated with six aromatic CKs were quantified using UPLC-MS/MS. The underground parts had higher endogenous CK levels than the aerial parts. Control plantlets had more isoprenoid CKs while the aromatic-type CKs were predominant in all other regenerants. Following acclimatization of the control and 10 渭M CK regenerants, there was a rapid decline in both isoprenoid and aromatic CK in the greenhouse-grown plants. Apart from the control and 6-(3-Methoxybenzylamino)-9-tetrahydropyran-2-ylpurine (MemTTHP) treatment with higher level of isoprenoid CK, aromatic CK remain the predominant CK-type across all CK treatments. The most abundant CK forms were meta-topolin (mT) and benzyladenine (BA) in the micropropagated and acclimatized plants, respectively. Micropropagated plantlets had cis-Zeatin (cZ) as the major isoprenoid CK-type which was in turn replaced by isopentenyladenine (iP) upon acclimatization. On a structural and functional basis, 9-glucoside, a deactivation/detoxicification product was the most abundant and mainly located in the underground parts (micropropagation and acclimatization). The results establish the wide variation in metabolic products of the tested aromatic CKs during micropropagation and acclimatization. The findings are discussed with the possible physiological roles of the various CK constituents on the growth and development of banana plants.