The role of thiol-reducing agents on modulation of glutamate binding induced by heavy metals in platelets

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• 作者：V.C. Borges ; C.W. Nogueira
• 关键词：Platelets ; Heavy metals ; Glutamate binding ; Chelating agents
• 刊名：Toxicology in Vitro
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：22
• 期：2
• 页码：438-443
• 全文大小：282 K

文摘

In the present study, we investigated if thiol-reducing agents are capable of altering mercury (Hg²⁺), lead (Pb²⁺) and cadmium (Cd²⁺) effects on platelet glutamatergic system. Dimercaprol (BAL), a dithiol chelating agent therapeutically used for the treatment of heavy metals poisoning, was capable of protecting the [³H]-glutamate binding against the effects caused by Pb²⁺ and Hg²⁺. 2,3-Dimercaptopropane-1-sulfonic acid (DMPS), another dithiol-reducing chelating agent, was capable of protecting the effect caused by Cd²⁺, Pb²⁺ and Hg²⁺. The similar effect was observed with addition of dithiothreitol (DTT) on [³H]-glutamate binding in human platelets. Dithiol-reducing agents (BAL, DMPS and DTT) alone did not alter [³H]-glutamate binding. In contrast, reduced glutathione (GSH), a monothiol-reducing agent, caused a significant inhibition on [³H]-glutamate binding at all concentrations tested. GSH did not modify heavy metal effects on [³H]-glutamate binding in platelets. The findings of the present investigation indicate that dithiol-reducing agents are capable of altering Hg²⁺, Pb²⁺ and Cd²⁺ effects on platelet glutamatergic system. In vitro data on chelating 13;metal interactions provide only an estimated guide to the treatment of heavy metal poisoning. Consequently, more studies in intoxicated patients are necessary to determine the precise use of the peripheral models and chelating agents.