Measurement of concentrations of whole blood levels of choline, betaine, and dimethylglycine and their relations to plasma levels

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• 作者：Hussain Mohamad Awwad ^{s9huawwa@stud.uni-saarland.de" class="auth_mail} ; Susanne H. Kirsch ; Juergen Geisel ; Rima Obeid ; ^{rima.obeid@uniklinikum-saarland.de" class="auth_mail}
• 关键词：BHMT ; betaine-homocysteine methyltransferase ; CV ; coefficient of variation ; DMG ; dimethylglycine ; Hcy ; homocysteine ; IDL ; instrumental detection limit ; LOD ; limit of detection ; MRM ; multiple reaction monitoring ; RBC ; red blood cell ; tHcy ; total homocysteine ; UPLC&ndash ; MS/MS ; ultra performance liquid chromatography tandem mass spectrometry ; WB ; whole blood
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：15 April, 2014
• 年：2014
• 卷：957
• 期：Complete
• 页码：41-45
• 全文大小：712 K

文摘

We aimed at developing a method for the measurement of choline and its metabolites in whole blood (WB). After an extraction step, quantification of choline, betaine, and dimethylglycine (DMG) was performed using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Plasma and WB metabolites were evaluated in a group of 61 elderly people. The calibration curves were linear (r² > 0.997) for all compounds. The inter- and intra-assay coefficients of variation for all analytes were <10%. The recoveries were >90% and the relative matrix effect were 鈮?.0%. The median concentrations of choline, betaine, and DMG were 11.3, 27.8, and 5.9 渭mol/L in plasma and 66.6, 165, and 13.7 渭mol/L in WB, respectively. There were positive correlations between WB and plasma markers; for choline (r = 0.42), betaine (r = 0.61), and DMG (r = 0.56) (all p 鈮?#xA0;0.001). The concentrations of betaine in WB and plasma were significantly higher in men than in women. The concentrations of WB choline and DMG did not differ significantly according to sex. In conclusion, we have established a reliable method for measuring choline metabolites in WB. The concentrations of WB choline, betaine, and DMG seem to reflect intracellular concentrations of these metabolites.