The optimum conditions for freeze-drying of probiotic cells were assessed.
Cell counts up to 10.9 log cfu/g were observed applying 5 °C/min cooling rate.
Lactose was the most efficient lyoprotectant for immobilized cells on apples pieces.
Monosodium glutamate proved the best lyoprotectant for immobilized cells on casein.
After storage for 1 year at 4 °C, cell counts were higher than 6 log cfu/g.