Effect of cooling rate, freeze-drying, and storage on survival of free and immobilized Lactobacillus casei ATCC 393
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文摘

The optimum conditions for freeze-drying of probiotic cells were assessed.

Cell counts up to 10.9 log cfu/g were observed applying 5 °C/min cooling rate.

Lactose was the most efficient lyoprotectant for immobilized cells on apples pieces.

Monosodium glutamate proved the best lyoprotectant for immobilized cells on casein.

After storage for 1 year at 4 °C, cell counts were higher than 6 log cfu/g.

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