Quantitative analysis of pasireotide (SOM230), a cyclic peptide, in monkey plasma using liquid chromatography in combination with tandem mass spectrometry
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- 作者：Yunlin Fu ; Wenkui Li ; wenkui.li@novartis.com" class="auth_mail" title="E-mail the corresponding author ; Jimmy Flarakos ; Francis L.S. Tse
- 关键词：Pasireotide ; SOM230 ; LC&ndash ; MS/MS ; Quantification ; Monkey plasma ; SPE ; μElution
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：1008
- 期：Complete
- 页码：242-249
- 全文大小：1710 K
文摘
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An LC–MS/MS method was developed and validated for the quantification of SOM230.
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Adding TFA to the mobile phases improved the chromatography and assay carryover.
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The SPE elution solvent needed to be freshly prepared for optimal LC–MS/MS results.
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Direct injection of SPE extract after dilution minimized nonspecific binding impact.
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The validated method was successfully used in a TOX study for SOM230 in monkeys.