Cardioprotective effects of baicalein on heart failure via modulation of Ca^{2 +} handling proteins in vivo and in vitro

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• 作者：Fali Zhao^a ; Lu Fu^a ; ^{fulu320@126.com" class="auth_mail" title="E-mail the corresponding author} ; Wei Yang^b ; Yuhui Dong^a ; Jing Yang^a ; Shoubin Sun^c ; Yuling Hou^a
• 关键词：Baicalein ; Heart failure (HF) ; Myocardial remodeling ; Apoptosis ; Ca2  ; + handling proteins
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 January 2016
• 年：2016
• 卷：145
• 期：Complete
• 页码：213-223
• 全文大小：2478 K

文摘

Baicalein is a widely used Chinese herbal medicine extracted from Labiatae plants Scutellaria baicalensis Georgi's dry root, which has multiple pharmacological activities. However, the precise mechanism of baicalein against myocardial remodeling remains poorly understood. The aim of our study was to investigate the underlying mechanism of baicalein treatment in rats model of heart failure (HF) and rat myocardial cells (H9C2).

Main methods

HF model was established by abdominal aorta constriction in rats and incubation with 50 μM isoproterenol for 48 h in H9C2 cells. Various molecular biological experiments were performed to assess the effects of baicalein on cardiac function, myocardial remodeling, apoptosis and Ca^2 + handling proteins.

Key findings

In the present study, first we found that baicalein alleviated HF in vivo. Additionally, treatment with baicalein inhibited the myocardial fibrosis, restrained the expression and activity of MMP2 and MMP9, and suppressed apoptosis in heart tissue. Moreover, baicalein could inhibit the cardiac myocyte hypertrophy and apoptosis induced by isoproterenol in vitro. Finally we found that baicalein could modulate the expressions and activities of Ca^2 + handling proteins, including downregulation of phosphorylation of Ca^2 +/calmodulin-dependent protein kinase II (CaMKII) and expression of Na(+)/Ca(2 +)-exchangers (NCX1), upregulation of sarcoplasmic reticulum Ca(2 +) ATPase 2 (SERCA2) and ryanodine receptor 2 (RYR2). Baicalein also restrained the decreased SERCA activity induced by aortic banding.

Significance

Our studies suggested that baicalein alleviated myocardial remodeling and improved cardiac function via modulation of Ca^2 + handling proteins, which may be a potential phytochemical flavonoid for therapeutics of HF.