Preconditioning of mesenchymal stem cells with 2,4-dinitrophenol improves cardiac function in infarcted rats

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• 作者：Irfan Khan^a ; Anwar Ali^a ; ^b ; Muhammad Aleem Akhter^a ; Nadia Naeem^a ; Maqsood Ahmed Chotani^a ; ^c ; ^d ; Tuba Mustafa^a ; Asmat Salim^a ; ^{asmat.salim@iccs.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Myocardial infarction ; Gene expression ; Cell therapy ; Transplantation ; Regeneration ; DNP ; Cell survival ; Cardiac function
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：1 October 2016
• 年：2016
• 卷：162
• 期：Complete
• 页码：60-69
• 全文大小：1988 K

文摘

The aim of this study is to determine if preconditioning of bone marrow derived mesenchymal stem cells (MSCs) with 2,4-dinitrophenol (DNP) improves survival of transplanted stem cells in a rat model of myocardial infarction (MI), and to asses if this strategy has measurable impact on cardiac function.

Main methods

MSCs were preconditioned with DNP. In vitro cell adhesion assay and qRT-PCR were performed to analyze the expression of genes involved in cardiomyogenesis, cell adhesion and angiogenesis. MI was produced by occlusion of left anterior descending coronary artery. One million cells were transplanted by intramyocardial injection into the infarcted myocardium. Echocardiography was performed after two and four weeks of cellular transplantation. Hearts were harvested after four weeks and processed for histological analysis.

Key findings

DNP treated MSCs adhered to the surface more (p < 0.001) as compared to the normal MSCs. Gene expression levels were significantly upregulated in case of DNP treatment. The number of viable MSCs was more (p < 0.001) in animals that received DNP treated MSCs, leading to significant improvement in cardiac function. Histological analysis revealed significant reduction in scar formation (p < 0.001), maintenance of left ventricular wall thickness (p < 0.001), and increased angiogenesis (p < 0.01).

Significance

The study evidenced for the first time that MSCs preconditioned with DNP improved cardiac function after transplantation. This can be attributed to improved survival, homing, adhesion, and cardiomyogenic and angiogenic differentiation of DNP treated MSCs in vivo.