Application of an intracellular assay for determination of tenofovir-diphosphate and emtricitabine-triphosphate from erythrocytes using dried blood spots
详细信息 查看全文
- 作者：Jia-Hua Zhenga ; Caitlin Rowera ; Kevin McAllistera ; Jose Castillo-Mancillab ; Brandon Kleina ; Amie Meditzb ; 1 ; L. Anthony Guidaa ; Jennifer J. Kisera ; Lane R. Bushmana ; Peter L. Andersona ; Peter.Anderson@ucdenver.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：TFV-DP ; tenofovir-diphosphate ; FTC-TP ; emtricitabine-triphosphate ; PrEP ; pre-exposure HIV prophylaxis ; iPrEx ; iniciativa profilaxis pre-exposicion (PrEP initiative) study ; DOT ; direct observed therapy ; TDF-FTC ; tenofovir disoproxil fumarate-emtricitabine
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：122
- 期：Complete
- 页码：16-20
- 全文大小：388 K
文摘
- •
Validation for TFV-DP and FTC-TP in erythrocytes using dried blood spots (DBS).
- •
Precise extraction demonstrated using replicate clinical samples.
- •
Analyte stability over 1 year at −20 °C to −80 °C and up to 5 days at ambient.
- •
Measurement not impacted by punch location, spot volumes, and hematocrit range tested.
- •
The method has been implemented as a biomarker of adherence to TDF-FTC therapy