The use of real-time polymerase chain reaction with high resolution melting (real-time PCR-HRM) analysis for the detection and discrimination of nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus
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- 作者：Anna Filipiaka ; A.Filipiak@iorpib.poznan.pl" class="auth_mail" title="E-mail the corresponding author ; Beata Hasió ; w-Jaroszewskab
- 关键词：Bursaphelenchus xylophilus ; Bursaphelenchus mucronatus ; Real-time PCR-HRM ; Pine wilt disease ; Quarantine nematode ; Detection
- 刊名：Molecular and Cellular Probes
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：30
- 期：2
- 页码：113-117
- 全文大小：828 K
文摘
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The real-time PCR-HRM analysis was developed for the detection and discrimination of Bursaphelenchus xylophilus and Bursaphelenchus mucronatus.
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The results showed distinct melting curve profiles related to each of examined species.
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The method may be used to detect and discriminate these both species, regardless of the life stages of nematodes and template concentrations.