Development of a selective activity-based probe for glycosylated LIPA

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• 作者：Adam G. Schwaid^a ; ^{aschwaid@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Wanida Ruangsiriluk^b ; Allan R. Reyes^b ; Shawn Cabral^c ; Francis Rajamohan^d ; Meihua Tu^a ; Jessica Ward^b ; Philip A. Carpino^a
• 关键词：Lysosomal acid lipase ; Activity based protein profiling ; Serine hydrolase ; Glycosylation ; LAL ; LIPA
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2016
• 出版时间：15 April 2016
• 年：2016
• 卷：26
• 期：8
• 页码：1993-1996
• 全文大小：922 K

文摘

Loss of LIPA activity leads to diseases such as Wolman’s Disease and Cholesterol Ester Storage Disease. While it is possible to measure defects in LIPA protein levels, it is difficult to directly measure LIPA activity in cells. In order to measure LIPA activity directly we developed a LIPA specific activity based probe. LIPA is heavily glycosylated although it is unclear how glycosylation affects LIPA activity or function. Our probe is specific for a glycosylated form of LIPA in cells, although it labels purified LIPA regardless of glycosylation.