Amplified fluorescence detection of DNA based on catalyzed dynamic assembly and host-guest interaction between β-cyclodextrin polymer and pyrene
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- 作者：Haihua Huang ; Xiaohai Yang ; kmwang@hnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Kemin Wang ; yangxiaohai@hnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Qing Wang ; Qiuping Guo ; Jin Huang ; Jianbo Liu ; Xiaochen Guo ; Wenshan Li ; Leiliang He
- 关键词：Enzyme-free ; Pyrene ; β-cyclodextrin polymer ; Nucleic acid detection
- 刊名：Talanta
- 出版年：2015
- 出版时间：1 November 2015
- 年：2015
- 卷：144
- 期：Complete
- 页码：529-534
- 全文大小：877 K
文摘
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Our assay is based on the host–guest interaction between β-CDP and pyrene.
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The hairpin probe is easy to design, synthesize, purify and convenient for usage.
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The detection system relies on hybridization and strand displacement reactions.
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Our assay displays fine reproducibility and practical utility in complex samples.