刊名:Biochemical and Biophysical Research Communications
出版年:2017
出版时间:29 January 2017
年:2017
卷:483
期:1
页码:366-370
全文大小:654 K
卷排序:483
文摘
Mutation of Q-loop glutamines (Q475A/Q1118A) inhibited drug-stimulated ATPase activity in human P-glycoprotein. The Q475A/Q1118A mutations disrupted signaling between the transmembrane- and nucleotide-binding domains. Cross-linking of mutant L175C/N820C/Q475A/Q1118A with a flexible cross-linker restored drug-stimulated ATPase activity. Gln475 and Gln1118 form part of a spring-like mechanism required for open to closed conformational changes.