Immediate stabilization of human blood for delayed quantification of endogenous thiols and disulfides
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- 作者：Daniela Giustarinia ; Federico Galvagnib ; Maurizio Orlandinib ; Paolo Fantic ; Ranieri Rossia ; ranieri@unisi.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Alb ; albumin ; Cys ; cysteine ; CysGly ; Cysteinylglycine ; CyGlySSP ; protein mixed disulfides with CysGly ; CySS ; cystine ; CySS-Alb ; S-cysteinylated albumin ; CySSGly ; cystinylglycine ; CySSP ; S-cysteinylated proteins ; DTNB ; 5&prime ; -dithiobis-(2-nitrobenzoic acid) ; DTT ; dithiothreitol ; γ-GluCys ; γ-Glutamylcysteine ; GSH ; glutathione ; GSSG ; glutathione disulfide ; GSSP ; S-glutathionylated proteins ; Hb ; hemoglobin ; HbSSG ; S-glutathionylated hemoglobin ; Hcys ; homocysteine ; HcySS ; homocystine ; HcySSP ; S-hom
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：1019
- 期：Complete
- 页码：51-58
- 全文大小：1545 K
文摘
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A new procedure to stabilize thiols and disulfides in different blood fractions for months is proposed.
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N-Ethylmaleimide was used to stabilize thiols in whole blood, RBCs, platelets and disulfides in plasma.
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Addition of citrate buffer followed by dilution with H2O was used to stabilize plasma thiols.
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The concentration of thiols and disulfides resulted to be stable for 6 months in stored samples.
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Only 3 ml of blood were needed to measure thiols and disulfides in the different blood fractions.