Liposome-DNA interaction: microcalorimetric study
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- 作者：Mrevlishvili ; G.M. ; Kankia ; B.I. ; Mdzinarashvili ; T.J. ; Brelidze ; T.I. ; Khvedelidze ; M.M. ; et. al.
- 关键词：Liposomes ; DNA ; Phase transition ; DSC ; DPC ; 1 ; 2-Ditetradecanoyl-sn-glycero-3-phosphocholine ; TDTMHA-Br ; tetradecyltrimethylammonium bromide ; DSC ; differential scanning calorimetry
- 刊名：Chemistry and Physics of Lipids
- 出版年：1998
- 出版时间：July, 1998
- 年：1998
- 卷：94
- 期：1
- 页码：139-143
- 全文大小：76.9 K
文摘
The authors applied differential scanning calorimetry (DSC) for studying the thermodynamic characteristics of DNA-liposome interactions. At the first stage, the melting curves of the ‘order–disorder’ thermal transition for lipid component and of the ‘ helix-coil’ transition for DNA were obtained. At the second stage, the phase behavior of the DNA-lipid mixture as a function of both components (lipid/DNA ratio) was obtained. The liposome-DNA interaction was investigated comparing the melting curves of the pure components and the mixture.