Identification of an unusual type II thioesterase in the dithiolopyrrolone antibiotics biosynthetic pathway
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- 作者：Ying Zhaia ; Silei Baia ; Jingjing Liua ; Liyuan Yanga ; Li Hanb ; Xueshi Huangb ; Jing Hea ; hejingjj@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Dithiolopyrrolone ; Biosynthetic gene cluster ; Aureothricin ; Heterologous expression ; In-frame gene deletion ; Type II thioesterase
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：22 April 2016
- 年：2016
- 卷：473
- 期：1
- 页码：329-335
- 全文大小：1599 K
文摘
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Cloning of the aureothricin biosynthetic gene cluster from Streptomyces thioluteus DSM 40027.
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Identification of the aureothricin gene cluster by heterologous expression and in-frame gene deletion.
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The heterogenetic thioesterase HlmK significantly improved dithiolopyrrolones production of the aureothricin gene cluster.
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Identification of HlmK as an unusual type II thioesterase.