Reactive oxygen species induced by presynaptic glutamate receptor activation is involved in [³H]GABA release from rat brain cortical nerve terminals

详细信息    查看全文

• 作者：A. Tarasenko ; O. Krupko ; N. Himmelreich ; ^{ninahimm@biochem.kiev.ua}
• 关键词：GABA release ; Kainate ; NADPH oxidase ; NMDA ; Pyruvate ; ROS
• 刊名：Neurochemistry International
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：61
• 期：7
• 页码：1044-1051
• 全文大小：609 K

文摘

We investigated the production of reactive oxygen species (ROS) as a response to presynaptic glutamate receptor activation, and the role of ROS in neurotransmitter (GABA) release. Experiments were performed with rat brain cortical synaptosomes using glutamate, NMDA and kainate as agonists of glutamate receptors. ROS production was evaluated with the fluorogenic compound dichlorodihydrofluorescein diacetate (H₂DCF-DA), and GABA release was studied using synaptosomes loaded with [³H]GABA. All agonists were found to stimulate ROS production, and specific antagonists of NMDA and kainate/AMPA receptors, dizocilpine hydrogen maleate (MK-801) and 6-cyano-7-nitroquinoxaline-2,3-done (CNQX), significantly inhibited the ROS increase. Spontaneous as well as agonist-evoked ROS production was effectively attenuated by diphenyleneiodonium (DPI), a commonly used potent inhibitor of NADPH oxidase activity, that suggests a high contribution of NADPH-oxidase to this process. The replacement of glucose with pyruvate or the simultaneous presence of both substrates in the medium led to the decrease in spontaneous and NMDA-evoked ROS production, but to the increase in ROS production induced by kainate. Scavenging of agonist-evoked ROS production by a potent antioxidant N-acetylcysteine was tightly correlated with the inhibition of agonist-evoked GABA release. Together, these findings show that the activation of presynaptic glutamate receptors induces an increase in ROS production, and there is a tight correlation between ROS production and GABA secretion. The pivotal role of kainate/AMPA receptors in ROS production is under discussion.