A biotinylated intercalator for selective post-labeling of double-stranded DNA as a basis for high-sensitive DNA assays

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• 作者：Magdalena Gę ; bala ; Leonard Stoica ; Dmitrii Guschin ; Lutz Stratmann ; Gerhard Hartwich ; Wolfgang Schuhmann
• 关键词：Biotinylated proflavine ; Intercalation ; Label-free DNA detection ; DNA hybridization ; Electrochemical impedance spectroscopy ; Enzyme amplification
• 刊名：Electrochemistry Communications
• 出版年：2010
• 出版时间：May 2010
• 年：2010
• 卷：12
• 期：5
• 页码：684-688
• 全文大小：436 K

文摘

For increasing the sensitivity of label-free DNA assays an amplification strategy is proposed based on the synthesis of a proflavine derivative which on the one hand retains its high affinity for double-stranded DNA (dsDNA) intercalation and on the other hand is functionalized via a flexible spacer with biotin moieties. By this, subsequent to the post-labeling of areas with dsDNA, reporter systems such as streptavidin/enzyme conjugates can be bound. Amplified DNA hybridization detection using an oligonucleotide model system, a biotinylated proflavine as intercalator and streptavidin/alkaline phosphatase is demonstrated.