MRI/optical dual-modality imaging of vulnerable atherosclerotic plaque with an osteopontin-targeted probe based on Fe₃O₄ nanoparticles

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• 作者：Hongyu Qiao^a ; ^b ; ¹ ; Yabin Wang^a ; ¹ ; Ruohan Zhang^c ; ¹ ; Quansheng Gao^f ; Xiao Liang^d ; Lei Gao^a ; Zhenhua Jiang^b ; Ruirui Qiao^e ; Dong Han^b ; Yan Zhang^a ; Ya Qiu^a ; Jie Tian^d ; Mingyuan Gao^e ; ^{gaomy@iccas.ac.cn" class="auth_mail" title="E-mail the corresponding author} ; Feng Cao^a ; ^b ; ^{wind8828@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dual-modality molecular imaging ; Atherosclerotic plaques ; Macrophages ; Nanoparticles
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：336-345
• 全文大小：2251 K

文摘

Rupture of vulnerable atherosclerotic plaque is the major pathological cause of luminal thrombosis in acute coronary syndromes. Since foamy macrophages have been identified as a prominent component in vulnerable atherosclerotic lesions and osteopontin (OPN) is reported to be highly expressed in foamy macrophages, OPN could be a potential target for vulnerable atherosclerotic plaque imaging. The current study designed an OPN-specific MRI/optical dual-modality probe to detect vulnerable plaques. Fluorescence imaging revealed that 24 h after injection of the Cy5.5-OPN-DMSA-MNPs (COD-MNPs), the atherosclerotic plaques in carotid artery exhibited significant higher signals in high fat diet (HFD) fed mice in comparison to the group injected with Cy5.5-IgG-DMSA-MNPs (CID-MNPs) or normal diet fed group injected with COD-MNPs (1.87 ± 0.19 × 10¹⁰ vs. 0.74 ± 0.04 × 10¹⁰, 0.73 ± 0.03 × 10¹⁰ p/sec/cm²/sr, P < 0.05). Meanwhile, MRI displayed stronger T₂ contrast enhancement 24 h post-injection at the area of atherosclerotic plaques in the carotid of HFD fed group injected with COD-MNPs than group injected with CID-MNPs or normal diet fed group injected with COD-MNPs (post/pre signal ratio: 0.64 ± 0.04 vs. 0.95 ± 0.02, 0.98 ± 0.01, P < 0.05). As a dual-modality molecular probe, the resulting COD-MNPs conjugates exhibit promising potentials for noninvasive detection of vulnerable atherosclerotic plaque in vivo.