DNA/protein binding, cytotoxicity and catecholase activity studies of a piperazinyl moiety ligand based nickel(II) complex

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• 作者：Soumen Mistri^a ; Horst Puschmann^b ; Subal Chandra Manna^a ; ^{scmanna@mail.vidyasagar.ac.in" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ni(II) complex ; Crystal structure ; DNA binding studies ; Serum albumin binding studies ; Catecholase activity
• 刊名：Polyhedron
• 出版年：2016
• 出版时间：5 September 2016
• 年：2016
• 卷：115
• 期：Complete
• 页码：155-163
• 全文大小：2121 K

文摘

The Ni(II) complex, {[Ni(HL)(SCN)₂(H₂O)]·2(DMF)} (1) [HL = 6-methoxy-2-{[2-(1-piperazinyl)ethylimino]methyl}phenol], was synthesized and characterized by X-ray crystal structure analysis and spectroscopic methods. The crystal structure of complex 1 reveals a distorted octahedral coordination geometry around the nickel centre which forms a supramolecular assembly through hydrogen bonds. The interaction of complex 1 with the calf thymus DNA (CT-DNA) was investigated using electronic absorption and fluorescence spectroscopic methods. The results show that complex 1 has binding affinity to CT-DNA in the order of 6.06 × 10⁵ M⁻¹. The interactions of complex 1 with bovine serum albumin (BSA) and human serum albumin (HSA) were also studied using electronic absorption and fluorescence spectroscopic techniques and the analysis show that interaction of complex 1 with BSA/HSA occur mainly with ground state association process. The number of binding sites and binding constant were calculated using double logarithm regression equation. Anticancer activity of 1 in human breast (MCF7) cancer cell lines reveals dose dependent suppression of cell viability with IC₅₀ value 64 ± 3.7 μM. Catecholase activity of 1 has been investigated in methanol medium using 3,5-di-tert-butylcatechol (3,5-DTBC) as model substrate and the result shows that 1 is active for catalyzing aerobic oxidation of 3,5-DTBC to 3,5-di-tert-butylbenzoquinone (3,5-DTBQ).