In vitro selection of RNA-cleaving DNAzymes for bacterial detection
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- 作者：Wenqing Zhanga ; Qian Fengb ; Dingran Changa ; Kha Trama ; Yingfu Lia ; liying@mcmaster.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：DNAzyme ; In vitro selection ; RNA cleavage ; Bacterial detection ; Biosensor ; Clostridium difficile
- 刊名：Methods
- 出版年：2016
- 出版时间：15 August 2016
- 年：2016
- 卷：106
- 期：Complete
- 页码：66-75
- 全文大小：1377 K
文摘
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RNA-cleaving DNAzymes are DNA-based enzymes for RNA cleavage.
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Bacteria-responding RNA-cleaving DNAzymes can be isolated from a random DNA pool.
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These DNAzymes can be used to set up simple assays for bacterial detection.