Inactivation of the VID27 gene prevents suppression of the doa4 degradation defect in doa4Δ did3Δ double mutant

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• 作者：Nazia Wolters^a ; Alexander Amerik^a ; ^b ; ^{amerik.alexander@gmail.com} ; ^{amerik@sky.chph.ras.ru}
• 关键词：Ubiquitin ; Deubiquitinating enzymes ; Vacuolar import and degradation ; Vacuolar protein sorting ; Doa4-independent degradation ; Class E compartment
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：1341-1345
• 全文大小：1111 K
• 卷排序：482

文摘

doa4Δ did3Δ double mutant cells efficiently degrading a short-lived reporter protein Deg1-βgal were mutagenized. Four colonies that lost the ability to degrade Deg1-βgal were isolated. Inactivation of the VID27 (vacuolar import and degradation) gene restores the doa4 phenotype in doa4Δ did3Δ double mutant.