Safe and efficient method for cryopreservation of human induced pluripotent stem cell-derived neural stem and progenitor cells by a programmed freezer with a magnetic field
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- 作者：Yuichiro Nishiyamaa ; b ; Akio Iwanamia ; Jun Kohyamab ; Go Itakurab ; Soya Kawabataa ; Keiko Sugaia ; Soraya Nishimuraa ; Rei Kashiwagia ; Kaori Yasutakea ; Miho Isodab ; c ; Morio Matsumotoa ; Masaya Nakamuraa ; masa@a8.keio.jp" class="auth_mail" title="E-mail the corresponding author ; Hideyuki Okanob ; hidokano@a2.keio.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cells Alive System (CAS) ; Magnetic field ; Cryopreservation ; Human iPSC-derived neural stem/progenitor cells (hiPSC-NS/PCs) ; Neurosphere ; Allogeneic transplantation ; Spinal cord injury (SCI) ; Central nervous system (CNS) disorder
- 刊名：Neuroscience Research
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：107
- 期：Complete
- 页码：20-29
- 全文大小：2537 K
文摘
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CAS cryopreservation improved cell survival immediately after thawing.
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CAS did not significantly affect proliferation, differentiation or gene expression.
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We found the optimal and useful freezing method of hiPSC-NS/PCs.
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This method enables us to apply allogeneic transplantation for SCI patients.