Identification of an Actin Binding Surface on Vinculin that Mediates Mechanical Cell and Focal Adhesion Properties
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- 作者：Peter M. Thompson1 ; 2 ; 7 ; Caitlin E. Tolbert3 ; 7 ; Kai Shen1 ; 8 ; Pradeep Kota1 ; 2 ; 9 ; Sean M. Palmer1 ; 2 ; 10 ; Karen M. Plevock1 ; 2 ; Albina Orlova4 ; Vitold E. Galkin4 ; 11 ; Keith Burridge3 ; 5 ; Edward H. Egelman4 ; Nikolay V. Dokholyan1 ; 5 ; Richard Superfine6 ; Sharon L. Campbell1 ; 5 ; campbesl@med.unc.edu" class="auth_mail
- 刊名：Structure
- 出版年：6 May 2014
- 年：2014
- 卷：22
- 期：5
- 页码：697-706
- 全文大小：2759 K
文摘
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Identification of a vinculin point mutant specifically impaired in actin binding
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An actin-binding surface on helix 4 of Vt is identified by negative-stain EM
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I997 and V1001 anchor the hydrophobic patch on Vt that binds F-actin
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Loss of actin-binding by vinculin impairs mechanotransduction