Photo-dynamics of photoactivated adenylyl cyclase TpPAC from the spirochete bacterium Turneriella parva strain HT
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- 作者：Alfons Penzkofera ; alfons.penzkofer@physik.uni-regensburg.de" class="auth_mail" title="E-mail the corresponding author ; Meenakshi Tanwarb ; Sindhu Kandoth Veetilb ; Suneel Kateriyac
- 关键词：Photoactivated adenylyl cyclase TpPAC from Turneriella parva ; BLUF domain photocycle ; cAMP cyclase activity ; Apparent protein melting temperature ; Photo-degradation ; Optogenetics
- 刊名：Journal of Photochemistry and Photobiology B: Biology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：153
- 期：Complete
- 页码：90-102
- 全文大小：1737 K
文摘
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Photoactivated adenylyl cyclase TpPAC from Turneriella parva was synthesized.
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Adenylyl cyclase activity of TpPAC in dark and light adapted states was measured.
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The primary photo-cycle dynamics of TpPAC BLUF domain was studied quantitatively.
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Quantum efficiency of BLUF domain signaling state formation was found to be 0.59.
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Photo-degradation of flavin in BLUF domain led to reduced flavin–protein adduct.