Photo-dynamics of BLUF domain containing adenylyl cyclase NgPAC3 from the amoeboflagellate Naegleria gruberi NEG-M strain
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- 作者：A. Penzkofera ; alfons.penzkofer@ur.de" class="auth_mail ; M. Tanwarb ; S.K. Veetilb ; S. Kateriyab ; M. Stierlc ; P. Hegemannc
- 关键词：Photo-activated cyclase ; BLUF domain ; Amoeboflagellate Naegleria gruberi ; Photo-cycle dynamics ; Apparent protein melting temperature ; Adenylyl cyclase activity
- 刊名：Journal of Photochemistry and Photobiology A: Chemistry
- 出版年：1 August 2014
- 年：2014
- 卷：287
- 期：Complete
- 页码：19-29
- 全文大小：1295 K
文摘
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Photo-activated adenylyl cyclase NgPAC3 from Naegleria gruberi NEG-M was expressed.
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Photo-cycle dynamics of BLUF domain in NgPAC3 protein was studied.
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Flavin cofactor is partly fully oxidized and partly fully reduced.
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Adenylyl cyclase activity was studied under dark and light adapted conditions.
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Thermal stability was studied and apparent melting temperature was determined.