Here is presented a
deficient paternity case requested by a son born out of wedlock. In or
der to avoid exhumation of the alleged father, the judge
deman
ded to obtain his biological profile from the analysis of his closest living relatives. Paternity testing involved the applicant (Sm), his mother (M2) and two sons born in wedlock of the
deceased father (S1 and S2). The analysis of 23 Y-STRs by means of the PowerPlex Y23 System revealed a full sharing of Y-chromosomal profiles between the applicant (Sm) and one son of the
deceased father (S2), but no compatibility with the second (S1), thus suggesting the presence of an additional alleged father.
Giving the impossibility to establish which between S1 and S2 was the true biological son and, consequently, which 23 Y-STR profile matched with the deceased father, the judge disposed exhumation of the body.
Full identity of Y-STRs was revealed among the deceased, S2 and Sm, whilst S1 could be excluded from the lineage.
A following calculation of Likelihood Ratios achieved from the analysis with 22 autosomal STRs (PowerPlex Fusion System) allowed complete solving of the paternity case. Sm-S2 were found to be half-brothers with the same father, S1-S2 half-brothers sharing a common mother and Sm-S1 were declared unrelated.