Mammalian complex I: A regulable and vulnerable pacemaker in mitochondrial respiratory function

详细信息    查看全文

• 作者：Sergio Papa ; Domenico De Rasmo ; Salvatore Scacco ; Anna Signorile ; Zuzana Technikova-Dobrova ; Giuseppe Palmisano ; Anna Maria Sardanelli ; Francesco Papa ; Damiano Panelli ; Raffaella Scaringi ; Arcangela S
• 关键词：Complex I ; Proton pump ; cAMP cascade ; PKA ; Mitochondrial import ; Mitochondrial phosphoproteome
• 刊名：Biochimica et Biophysica Acta (BBA)/Bioenergetics
• 出版年：2008
• 出版时间：July-August 2008
• 年：2008
• 卷：1777
• 期：7-8
• 页码：719-728
• 全文大小：1879 K

文摘

In this paper the regulatory features of complex I of mammalian and human mitochondria are reviewed. In a variety of mitotic cell-line cultures, activation in vivo of the cAMP cascade, or direct addition of cAMP, promotes the NADH–ubiquinone oxidoreductase activity of complex I and lower the cellular level of ROS. These effects of cAMP are found to be associated with PKA-mediated serine phosphorylation in the conserved C-terminus of the subunit of complex I encoded by the nuclear gene NDUFS4. PKA mediated phosphorylation of this Ser in the C-terminus of the protein promotes its mitochondrial import and maturation. Mass-spectrometry analysis of the phosphorylation pattern of complex I subunits is also reviewed.