Pectic polysaccharides of the fresh plum Prunus domestica L. isolated with a simulated gastric fluid and their anti-inflammatory and antioxidant activities

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• 作者：Sergey V. Popov ; Raisa G. Ovodova ; Victoria V. Golovchenko ; Daria S. Khramova ; Pavel A. Markov ; Vasily V. Smirnov ; Alex ; re S. Shashkov ; Yury S. Ovodov
• 关键词：Plums ; Pectin structure ; Simulated gastric fluid ; NMR spectroscopy of polysaccharides ; Leukocyte adhesion ; Superoxide anion radical scavenging
• 刊名：Food Chemistry
• 出版年：2014
• 出版时间：15 January, 2014
• 年：2014
• 卷：143
• 期：Complete
• 页码：106-113
• 全文大小：586 K

文摘

A pectic polysaccharide, designated as PD, was extracted from fresh plums (Prunus domestica L.) with a simulated gastric fluid. Galacturonan, which was partially substituted with methyl and O-acetyl ester groups, and rhamnogalacturonan were the main constituents of the linear regions of the sugar chains of PD. The ramified region contained mainly 1,4-linked ¦Â-d-galactopyranose residues and, to a lesser extent, 1,5-linked ¦Á-l-arabinofuranose residues. The separation of PD, by DEAE-cellulose column chromatography, yielded two pectic fractions: PD-1 and PD-2, eluted with 0.1 and 0.2 M NaCl, respectively. Enzymatic digestion of PD with 1,4-¦Á-d-polygalacturonase yielded the fraction PD-E. The parent pectin PD and the PD-1 fraction were found to diminish the adhesion of peritoneal leukocytes at the concentrations of 0.05-1.0 mg/ml. However, the PD-E fraction failed to have an effect on cell adhesion at the concentrations of 0.05-0.1 mg/ml. PD, PD-1 and PD-E were found to inhibit the production of superoxide anion radicals by reducing xanthine oxidase activity by 38 % , 97 % and 47 % , respectively. Therefore, the PD-1 fraction appeared to be an active fragment of pectic macromolecule isolated from fresh plum with a simulated gastric fluid.