Morphology and 18S rDNA gene sequence of Blepharisma sinuosum Sawaya, 1940 (Ciliophora: Heterotrichea) from Brazil

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• 作者：Noemi Mendes Fern ; es ; Roberto J¨²nio Pedroso Dias ; Marcus Vinicius Xavier Senra ; Carlos Augusto Gomes Soares ; In¨¢cio Domingos da Silva Neto
• 关键词：18S rDNA ; Blepharisma ; Brazil ; Ciliophora ; Macronucleus ; Morphology
• 刊名：European Journal of Protistology
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：49
• 期：4
• 页码：623-633
• 全文大小：4725 K

文摘

The morphology and morphometric data of seven populations of Blepharisma sinuosum from southeastern Brazil were investigated. The description is based on live observations, protargol impregnation, and scanning electron microscopy. Blepharisma sinuosum measures 75-255 ¦Ìm in length and 25-93 ¦Ìm in width and has a spindle-shaped body, pink color, a single contractile vacuole located at the posterior end, 50 adoral membranelles, a conspicuous paroral, 17-35 somatic kineties, a moniliform macronucleus with 2-7 connected nodules, and 3-20 micronuclei. Morphological comparisons with similar species were performed and suggest that B. americanum is the junior synonym of B. sinuosum. The 18S rDNA gene sequence of B. sinuosum was obtained and compared with that of other Blepharisma species. The length and GC content of the obtained sequence is 1652 bp and 47.03 % , respectively, and has a very high structural similarity (99.9 % ) with the B. undulans sequence. The validity of the classification of Blepharisma species in morphonuclear subgenera is also discussed.