Effects of angiotensin II and antagonists on AT₁ receptor expression in mesangial cells

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• 作者：Chansel ; Dominique ; Vandermeersch ; Sophie ; Llorens-Cortes ; Catherine ; Ardaillou ; Raymond
• 关键词：Angiotensin II receptor ; (Rat) ; Mesangial cell ; Angiotensin II receptor antagonist ; Dexamethasone
• 刊名：European Journal of Pharmacology
• 出版年：1999
• 出版时间：November 19, 1999
• 年：1999
• 卷：384
• 期：2-3
• 页码：223-230
• 全文大小：688 K

文摘

Rat mesangial cells were exposed to angiotensin II, angiotensin AT₁ receptor antagonists such as losartan, EXP 3174 and candesartan, or dexamethasone for increasing periods (1–24 h). Angiotensin AT_1A and AT_1B receptor mRNA were measured by reverse transcription-polymerase chain reaction (RT-PCR). Angiotensin II, losartan and EXP 3174 did not modify significantly angiotensin AT_1A and AT_1B receptor mRNA. Candesartan increased angiotensin AT_1B receptor mRNA and, to a lesser extent, angiotensin AT_1A receptor mRNA. In contrast, dexamethasone decreased mainly angiotensin AT_1B receptor mRNA. As shown by Western blot analysis, exposure of mesangial cells to angiotensin II, losartan or EXP 3174 did not produce any change in angiotensin AT₁ receptor protein, whereas dexamethasone and candesartan exerted inhibitory effects. In conclusion, the angiotensin AT_1B receptor subtype, the most abundantly distributed in rat mesangial cells, is inhibited by glucocorticoids. The effect of candesartan is more complex with a slight stimulation of angiotensin AT_1B mRNA and a marked inhibition of angiotensin AT₁ receptor protein. In contrast, angiotensin II and the other angiotensin AT₁ receptor antagonists studied are inactive on angiotensin AT₁ mRNA and protein.