A new homolog of FocA transporters identified in cadmium-resistant Euglena gracilis

详细信息    查看全文

• 作者：Claudine Delomé ; nie ; Emilie Foti ; Enora Floch ; Vimala Diderot ; Dominique Porquet ; Corinne Dupuy ; Jacqueline Bonaly
• 关键词：Euglena gracilis ; Xenobiotics ; Cadmium ; Resistance ; Gene overexpression
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：29 June 2007
• 年：2007
• 卷：358
• 期：2
• 页码：455-461
• 全文大小：1351 K

文摘

To better understand the cellular mechanism of stress resistance to various pollutants (cadmium, pentachlorophenol), we undertook a survey of the Euglena gracilis transcriptome by mRNA differential display and cDNA cloning. We performed a real-time RT-PCR analysis upon four selected genes. One of them significantly changed its expression level in response to stress treatments: B25 gene was overexpressed in Cd-resistant cells whereas it was down-regulated in PCP-adapted cells. By Race assays we obtained for B25 a 1093 bp cDNA. The deduced protein was identified as a bacterial formate/nitrite transporter (FocA) homolog and the gene was named EgFth. From all the data, we concluded that EgFth overexpression was related to chronic exposure to cadmium.