Cytoplasmic phospholipase A2 expression in human colon adenocarcinoma is correlated with cyclooxygenase-2 expression and contributes to prostaglandin E2 production

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• 作者：Valentine Panel ; Pierre-Yves Boë ; lle ; Jesus Ayala-Sanmartin ; Anne-Marie Jouniaux ; Richard Hamelin ; Joë ; lle Masliah ; Germain Trugnan ; Jean-Franç ; ois Flé ; jou and Dominique Wendum
• 关键词：Colon cancer ; Phospholipase A2 ; Prostaglandin E2 ; Cyclooxygenase-2
• 刊名：Cancer Letters
• 出版年：2006
• 出版时间：18 November 2006
• 年：2006
• 卷：243
• 期：2
• 页码：255-263
• 全文大小：213 K

文摘

Cytoplasmic phospholipase A2 (cPLA2) has a key role in prostaglandin production. The role of cPLA2 in intestinal tumorigenesis has been suggested, however, contradictory data are found in the literature. We evaluated cPLA2 and cyclooxygenase-2 (COX-2) protein expression in 65 colon carcinomas by immunohistochemistry, and in eight colorectal cancer cell lines by Western Blot. PGE2 production was evaluated by enzyme-immunoassay in the cell lines. We demonstrate that cPLA2 is overexpressed in approximately 50 % of colon cancers and cell lines. cPLA2 expression is correlated with COX-2 expression. Both cPLA2 and COX-2 expressions are important in regard to PGE2 production. Our data suggest that cPLA2 might be involved in colon tumor development.