Analytical evaluation of Sysmex UF-1000i for flow cytometric analysis of peritoneal fluid

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• 作者：Sabrina Buoro ; Rosanna Gustinetti ; Paola Dominoni ; Maria Grazia Alessio ; Patrizia Filisetti ; Maria Grazia Luc¨¤ ; Stefano Fagiuoli ; Cosimo Ottomano ; Giuseppe Lippi
• 关键词：Peritoneal fluid ; Flow cytometry ; Analytical performance ; Evaluation
• 刊名：Clinical Biochemistry
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：45
• 期：15
• 页码：1263-1265
• 全文大小：399 K

文摘

Objective

To evaluate analytical performance of Sysmex UF-1000i for peritoneal fluid analysis.

Methods

Functional sensitivity, imprecision, linearity and comparison studies were performed on peritoneal fluids.

Results

Total imprecision was 1.6-4.7 % , functional sensitivity 27/¦ÌL for white blood cell (WBC) and 32/¦ÌL for total nucleated cell (TNC) count. Linearity was excellent up to 983 cell/¦ÌL, carry-over < 0.2 % , correlation with manual microscopy always greater than 0.992.

Conclusion

The instrument exhibited optimal performance at the conventional WBC diagnostic thresholds.