Emergence of artemisinin-resistant malaria on the western border of Thailand: a longitudinal study

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• 作者：Aung Pyae Phyo ; MD^a ; ^b ; Standwell Nkhoma ; PhD^c ; Kasia Stepniewska ; PhD^a ; ^d ; ^e ; Elizabeth A Ashley ; MD^a ; ^b ; ^d ; Shalini Nair ; MSc^c ; Rose McGready ; MD^a ; ^b ; ^d ; Carit ler Moo^b ; Salma Al-Saai ; MSc^c ; Arjen M Dondorp ; MD^a ; ^d ; Khin Maung Lwin ; MD^b ; Pratap Singhasivanon ; MD^a ; Nicholas PJ Day ; FRCP^a ; ^d ; Nicholas J White ; FRS^a ; ^d ; Tim JC Anderson ; PhD^c ; Prof Franç ; ois Nosten ; MD^a ; ^b ; ^d ; ^{francois@tropmedres.ac}
• 刊名：The Lancet
• 出版年：2012
• 出版时间：26 May-1 June, 2012
• 年：2012
• 卷：379
• 期：9830
• 页码：1960-1966
• 全文大小：418 K

文摘

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Summary

Background

Artemisinin-resistant falciparum malaria has arisen in western Cambodia. A concerted international effort is underway to contain artemisinin-resistant m>Plasmodium falciparumm>, but containment strategies are dependent on whether resistance has emerged elsewhere. We aimed to establish whether artemisinin resistance has spread or emerged on the Thailand-Myanmar (Burma) border.

Methods

In malaria clinics located along the northwestern border of Thailand, we measured six hourly parasite counts in patients with uncomplicated hyperparasitaemic falciparum malaria (? % infected red blood cells) who had been given various oral artesunate-containing regimens since 2001. Parasite clearance half-lives were estimated and parasites were genotyped for 93 single nucleotide polymorphisms.

Findings

3202 patients were studied between 2001 and 2010. Parasite clearance half-lives lengthened from a geometric mean of 2¡¤6 h (95 % CI 2¡¤5-2¡¤7) in 2001, to 3¡¤7 h (3¡¤6-3¡¤8) in 2010, compared with a mean of 5¡¤5 h (5¡¤2-5¡¤9) in 119 patients in western Cambodia measured between 2007 and 2010. The proportion of slow-clearing infections (half-life ?¡¤2 h) increased from 0¡¤6 % in 2001, to 20 % in 2010, compared with 42 % in western Cambodia between 2007 and 2010. Of 1583 infections genotyped, 148 multilocus parasite genotypes were identified, each of which infected between two and 13 patients. The proportion of variation in parasite clearance attributable to parasite genetics increased from 30 % between 2001 and 2004, to 66 % between 2007 and 2010.

Interpretation

Genetically determined artemisinin resistance in m>P falciparumm> emerged along the Thailand-Myanmar border at least 8 years ago and has since increased substantially. At this rate of increase, resistance will reach rates reported in western Cambodia in 2-6 years.

Funding

The Wellcome Trust and National Institutes of Health.