Sequence variation in the promoter region of the cholinergic receptor muscarinic 3 gene and asthma and atopy

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• 作者：Joseph Donfack ; Paul Kogut ; Sean Forsythe ; Julian Solway ; Carole Ober
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2003
• 出版时间：March 2003
• 年：2003
• 卷：111
• 期：3
• 页码：527-532
• 全文大小：88 K

文摘

Background: Muscarinic acetylcholine receptors are members of the superfamily of G protein–coupled, 7 transmembrane– spanning proteins. They are important in the development of airway hyperresponsiveness. In the lung the M₃ receptor, encoded by the cholinergic receptor muscarinic 3 gene, is present in airway smooth muscle and mediates smooth muscle contraction. Objective: We considered the cholinergic receptor muscarinic 3 gene as a possible candidate gene for bronchial asthma and initiated studies to identify polymorphisms in the promoter region. Method: We identified 4 single-nucleotide polymorphisms (−708A/G, −627G/C, −513C/A, and −492C/T) and 2 short tandem repeat polymorphisms, a tetranucleotide (CTTT)_12-20 and a dinucleotide (GT)_6-19 repeat. Results: None of the identified single nucleotide polymorphisms were significantly more frequent in asthmatic patients (n = 76) compared with in healthy control subjects (n = 81). Furthermore, there was no evidence for nonrandom transmission of short tandem repeat polymorphism haplotypes to individuals with asthma or bronchial hyperresponsiveness (P > .50) in a large Hutterite pedigree. However, there was significant nonrandom transmission of haplotypes to individuals with skin test reactivity to cockroach allergens (global transmission disequilibrium test: χ² = 38.55, P = .013). Conclusions: These results suggest a possible role for this gene in atopic disorders. (J Allergy Clin Immunol 2003;111:527-32.)