Evaluation of DNAstable鈩?for DNA storage at ambient temperature

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• 作者：Susanne E. Howlett ; Hilda S. Castillo ; Lora J. Gioeni ; James M. Robertson ; Joseph Donfack
• 关键词：Forensic science ; Long-term DNA storage ; DNAstable&trade ; Room-temperature storage ; qPCR ; STRs
• 刊名：Forensic Science International: Genetics
• 出版年：January, 2014
• 年：2014
• 卷：8
• 期：1
• 页码：170-178
• 全文大小：1393 K

文摘

Preserving DNA is important for validation of prospective and retrospective analyses, requiring many expensive types of equipment (e.g., freezers and back-up generators) and energy. While freezing is the most common method for storing extracted DNA evidence or well-characterized DNA samples for validation studies, DNAstable鈩?(Biomatrica^庐), a commercially available medium for room temperature storage of DNA extracts was evaluated in this study. Two groups of samples consisting of different DNA quantities were investigated, one ranging from 20 to 400 ng (group 1) and the other one ranging from 1.4 to 20 ng (group 2). The DNA samples with and without DNAstable鈩?were stored at four different temperatures [鈭?5 掳C (room temperature), 鈭?0 掳C, 37 掳C or 50 掳C]. DNA degradation over several months was monitored by SYBR^庐 Green-based qPCR assays and by PCR amplification of the core CODIS STR markers for group 1 and 2 DNA samples, respectively. For the time points tested in this study (up to 365 days), the findings indicate that the 鈭?0 掳C controls and the DNAstable鈩?protected samples at room temperature provided similar DNA recoveries that were higher compared to the unprotected controls kept at RT, 37 掳C or 50 掳C. These results suggest that DNAstable鈩?can protect DNA samples with effectiveness similar to that of the traditional 鈭?0 掳C freezing method. In addition, extrapolations from accelerated aging experiments conducted at high temperatures support that DNAstable鈩?is an effective technology for preserving purified DNA at room temperature with a larger protective impact on DNA samples of low quantity (<20 ng).