Endothelial dysfunction after exposure to cobalt chloride enhanced vascular contractility

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• 作者：YuJin Seong ; EunHee Kim ; Tae-Gyu Park ; YoungMi Seok ; Woonyi Baek ; Si-Oh Kim ; Dong Gun Lim ; Dong Heon Yang and InKyeom Kim
• 关键词：Cobalt chloride ; Contraction ; Endothelial dysfunction ; Nitric oxide synthase ; Superoxide anion
• 刊名：Environmental Toxicology and Pharmacology
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：20
• 期：2
• 页码：297-304
• 全文大小：472 K

文摘

Brief exposure to cobalt chloride augmented vascular contractility. We hypothesized that endothelial dysfunction plays a role in the augmentation of aortic contractility, after brief exposure to cobalt chloride.

Rat aortic ring preparations were mounted in organ baths, exposed to cobalt chloride (0.3–300 μmol/L) for 30 min, and then subjected to contractile agents or relaxants 1 and 5 h after the end of exposure. Presence of cobalt chloride did not affect the contractile response to phenylephrine. Brief exposure to cobalt chloride, however, even at 5 h after the end of exposure, not only augmented contractile responses to KCl or phenylephrine but also attenuated the relaxant response to acetylcholine. The mechanical denudation of endothelium or inhibition of endothelial nitric oxide synthase with 100 μmol/L N^ω-nitro-l-arginine methyl ester abolished the augmentation of contractile responses. Pre-treatment with 150 units/mL of superoxide dismutase also abrogated the augmented contractile responses. Brief exposure to cobalt chloride did not affect the contractile response to phorbol dibutyrate in the presence or absence of calcium, or the expression of HSP70.

In conclusion, endothelial dysfunction plays an important role in the augmentation of aortic contractility, after brief exposure to cobalt chloride.