刊名:Biochimica et Biophysica Acta (BBA) - General Subjects
出版年:2017
出版时间:January 2017
年:2017
卷:1861
期:1
页码:2934-2941
全文大小:1049 K
卷排序:1861
文摘
Recombinant Alg1 glycosyltransferase was purified in high yield. A novel quantitative assay was established to study Alg1 β-1, 4 mannosyltransferase activity. These methods were applied to measure the effect on Alg1 activity of mutations identified in human ALG1-CDG patients. These new methods open the possibility to scale up DLO product PPGn2-Man for further downstream enzymatic reactions.