A new adsorption-elution technique for the concentration of aquatic extracellular antibiotic resistance genes from large volumes of water
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- 作者：Da-Ning Wang ; Lu Liu ; Zhi-Gang Qiu ; Zhi-Qiang Shen ; Xuan Guo ; Dong Yang ; Jing Li ; Wei-li Liu ; Min Jin ; jinminzh@126.com" class="auth_mail" title="E-mail the corresponding author ; Jun-Wen Li ; junwen9999@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Nucleic acid absorption particles ; Adsorption-elution technique ; Extracellular antibiotic resistance genes ; Concentration
- 刊名：Water Research
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：92
- 期：Complete
- 页码：188-198
- 全文大小：2607 K
文摘
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We present a technique for eARG recovery with 104 times more sensitive than qPCR.
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With our method, eARGs can be concentrated from large volume of water.
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We also describe novel nucleic acid adsorption particles with high capacity for eARGs.
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We determine eARGs concentration in natural water.