Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell
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- 作者：Ruirui Fenga ; b ; 1 ; Bo Liangb ; c ; 1 ; Chuantao Houb ; 1 ; Dongfei Hanb ; Lei Hanb ; c ; Qiaolin Langb ; Aihua Liub ; c ; liuah@qibebt.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Lihui Hana ; lhhan@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Homology modeling ; Xylose dehydrogenase mutants ; Thermostability ; Microbial surface display ; Xylose/O2 based biofuel cell
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：84
- 期：Complete
- 页码：78-85
- 全文大小：2019 K
文摘
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The simulated structure of XDH was obtained using homology modeling.
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XDH mutants were displayed on the surface of E. coli .
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The whole cell biocatalyst of mutated XDH showed excellent stability.
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XDH mutant exhibited enhanced stability in xylose/O2 biofuel cell.