Comparative proteomics analysis of apoptotic Spodoptera frugiperda cells during p35 knockout Autographa californica multiple nucleopolyhedrovirus infection
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- 作者：Qian Yu ; qianyucn@126.com" class="auth_mail" title="E-mail the corresponding author ; Youhua Xiong ; Jianliang Liu ; Qin Wang ; Yuanxin Qiu ; Dongling Wen
- 关键词：AcMNPV ; Autographa californica multiple nucleopolyhedrovirus ; ITRAQ ; isobaric tags for relative and absolute quantitation ; SCX ; strong cationic-exchange ; MRM ; multiple reaction monitoring ; GO ; gene ontology
- 刊名：Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：18
- 期：Complete
- 页码：21-29
- 全文大小：1061 K
文摘
Infection with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) mutants lacking a functional p35 gene can induce host cell apoptosis, which provides the possibility to use the potential of these viruses in the biological control of pest insects. Nonetheless, the proteomics or the protein changes of Spodoptera frugiperda (Sf9) cells infected with p35 knockout AcMNPV have not yet been studied. To further improve the use of AcMNPV, we set out to analyze the protein composition and protein changes of Sf9 cells of different infection stages by isobaric tag for relative and absolute quantification (iTRAQ) techniques. A total of 4004 sf9 proteins were identified by iTRAQ. After comparation of the significantly expressed 483 proteins from p35koAcMNPV-infected Sf9 cells and the significantly expressed 413 proteins from wtAcMNPV-infected Sf9 cells, we found that 226 proteins were specific to p35koAcMNPV-infected Sf9 cells. The 226 proteins were categorized according to GO classification for insects and were categorized into: biological processes, molecular functions and cellular components. Of interest, the most up-regulated proteins related to Epstein–Barr virus infection, RNA transport, Calcium signaling pathway, cGMP-PKG signaling pathway, oxidative phosphorylation and N-Glycan biosynthesis. Determination of the protein changes in p35 knockout AcMNPV-infected Sf9 cells would facilitate the better use of this virus-host cell interaction in pest insect control and other related fields.