Seamless deletion of a large DNA fragment in the taxol-producing fungus Pestalotiopsis microspora

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• 作者：Longfei Chen^a ; Yingying Li^a ; Qian Zhang^a ; Oren Akhberdi^a ; Dongsheng Wei^a ; Jiao Pan^a ; Xudong Zhu^a ; ^b ; ^{zhu11187@bnu.edu.cn}
• 关键词：ATMT ; 5-FOA ; Counter selection ; Orotidine 5&prime ; -phosphate decarboxylase ; Seamless genome reduction
• 刊名：Mycoscience
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：58
• 期：1
• 页码：35-39
• 全文大小：832 K
• 卷排序：58

文摘

A genome reduction method was developed and tested in Pestalotiopsis microspora. Gateway/overlap extension PCR were applied to avoid tedious plasmid operations. Two adjacent DNA sequences were sequentially removed in a single transformation step. The deletion cassette can be transformed by ATMT with high efficiency. A large genomic region was deleted with one construct, with no residual junk DNA.