Cellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma ¦Â-cells

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• 作者：Ruiguo Yang ; MEng^a ; Ning Xi ; DSc^a ; ^b ; ^{xin@egr.msu.edu} ; King Wai Chiu Lai ; PhD^a ; ^b ; Kevin C. Patterson ; BS^c ; Hongzhi Chen ; PhD^a ; Bo Song ; MEng^a ; Chengeng Qu ; BS^a ; Beihua Zhong ; MD^c ; ^d ; ^e ; Donna H. Wang ; MD^c ; ^d ; ^f ; ^{donna.wang@hc.msu.edu}
• 关键词：Ion channel activities ; AFM nanorobotics ; Cellular stiffness ; Insulinoma ¦Â-cells
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：9
• 期：5
• 页码：636-645
• 全文大小：2007 K

文摘

Distinct biochemical, electrochemical and electromechanical coupling processes of pancreatic ¦Â-cells may well underlie different response patterns of insulin release from glucose and capsaicin stimulation. Intracellular Ca^2 + levels increased rapidly and dose-dependently upon glucose stimulation, accompanied with about threefold rapid increases in cellular stiffness. Subsequently, cellular stiffness diminished rapidly and settled at a value about twofold of the baseline. Capsaicin caused a similar transient increase in intracellular Ca^2 + changes. However, cellular stiffness increased gradually to about twofold until leveling off. The current study characterizes for the first time the biophysical properties underlying glucose-induced biphasic responses of insulin secretion, distinctive from the slow and single-phased stiffness response to capsaicin despite similar changes in intracellular Ca^2 + levels. The integrated AFM nanorobotics and optical investigation enables the fine dissection of mechano-property from ion channel activities in response to specific and non-specific agonist stimulation, providing novel biomechanical markers for the insulin secretion process.

From the Clinical Editor

This study characterizes the biophysical properties underlying glucose-induced biphasic responses of insulin secretion. Integrated AFM nanorobotics and optical investigations provided novel biomechanical markers for the insulin secretion process.