P34^cdc2 kinase is associated with cortical microtubules from higher plant protoplasts

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• 作者：Hemsley ; Rachel ; McCutcheon ; Sandra ; Doonan ; John ; et. al.
• 关键词：PSTAIRE ; cdc2 ; Microtubule ; Plant cell ; Cytoskeleton ; PPB ; preprophase band ; CDK ; cyclin-dependent kinase ; MAP ; microtubule-associated protein
• 刊名：FEBS Letters
• 出版年：2001
• 出版时间：November 9, 2001
• 年：2001
• 卷：508
• 期：1
• 页码：157-161
• 全文大小：513 K

文摘

The cell cycle regulatory enzyme p34^cdc2 kinase is known to be localized to the preprophase band, the spindle and the phragmoplast, but not to interphase cortical microtubules. This was investigated further by mechanically cleaving substrate-attached protoplasts to leave plasma membrane disks bearing microtubules freed of nuclear and cytosolic signal. Antibodies to PSTAIRE and to specific C-terminal peptides of cdc2a, were used in immunofluorescence, protein blotting and immunogold electron microscopy to demonstrate that antigen is located on the cortical microtubules of carrot, tobacco BY-2 and Arabidopsis cells.