Functional expression of P2 purinoceptors in a primary neuroglial cell culture of the rat arcuate nucleus
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- 作者：Eric Pollatzek&dagger ; ; eric-pollatzek@gmx.de" class="auth_mail" title="E-mail the corresponding author ; Norma Hitzel&Dagger ; ; norma.hitzel@idt-biologika.de" class="auth_mail" title="E-mail the corresponding author ; Daniela Ott daniela.ott@vetmed.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author ; Katrin Raisl katrin.raisl@vetmed.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author ; Bä ; rbel Reuter¶ ; ; baerbel.reuter@physiologie.med.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author ; Rü ; diger Gerstberger ; ruediger.gerstberger@vetmed.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：[35S]dATPαS ; [α-35S]deoxyadenosine-5&prime ; thiotriphosphate ; [Ca2+]i ; intracellular calcium concentration ; 2-MeSADP ; 2-methylthioadenosine-5&prime ; diphosphate ; 2-MeSATP ; 2-methylthioadenosine-5&prime ; triphosphate ; αβ-meATP ; α ; β-methyleneadenosine-5&prime ; triphosphate ; ACTH ; adrenocorticotropin ; ARC ; arcuate nucleus ; ATP ; adenosine-5&prime ; triphosphate ; BSA ; bovine serum albumin ; BT ; body core temperature ; DA ; dopamine ; DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; DMH ; dorsomedial hypothalamus ; EGT
- 刊名：Neuroscience
- 出版年：2016
- 出版时间：7 July 2016
- 年：2016
- 卷：327
- 期：Complete
- 页码：95-114
- 全文大小：2804 K
文摘
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Establishment of a differentiated neuroglial primary cell culture system of the early postnatal rat arcuate nucleus (ARC).
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Calcium imaging experiments indicate expression of P2X2 and P2Y1 purinoceptors in ARC neurons and/or astrocytes.
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ARC neurons and astrocytes can be subdivided into partially temperature-dependent low and high desensitization groups.
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Competitive binding assays with the radioligand [35S]dATPαS support functional purinoceptor expression in rat hypothalamus.